SCHOTT North America

FAQ - Aminosilane coated Slides

Can SCHOTT recommend any blocking protocols for the NEXTERION^® aminosilane slides that do not involve BSA?

Yes, it is possible to chemically block the surface of NEXTERION^® Slide A+ and Astar. Unfortunately in most cases, the results are inferior to the BSA blocking method.
For this reason, we removed the method from our standard protocols some time ago. However, if you wish to try it, here are details of the chemical blocking for NEXTERION^® A+/AStar, it should take 20 minutes:

Reagents:
Amino Blocking Solution: 5 g succinic anhydride + 315 ml n-methylpyrrolidone + 35 ml 0.2 M sodium-borate solution pH 8. Add sodium borate freshly before use.

Protocol:

1 x 15 min incubation in Amino Blocking Solution at room temperature
1 x 10 to 20 sec washing in 0.1% SDS at room temperature, move slides up and down vigorously
1 x 10 to 20 sec washing in diH₂O at room temperature, move slides up and down vigorously
(Denaturing step for arrays spotted with PCR-probes) 1 x 3 min in boiling diH₂O
Dry the arrays in an oil-free air, or nitrogen stream, or by centrifugation (200 x g for 5 min) to avoid any water stains on the slide surface

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For printing DNA oligonucleotides onto NEXTERION^® aminosilane slides (Slides A / A+ / A*), your protocols state that I can use 50% DMSO. What should I dilute the DMSO with?

If your oligos are already in a buffer, the most usual procedure is to mix them with DMSO and ddH₂O to a final concentration of 1×print buffer / 50% DMSO.

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