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General information coated slides

Introduction


SCHOTT offers a wide range of functional coating chemistries for DNA and protein microarraying.
Nexterion® coated slides are available with a standard functional coating, or also with an additional reflective dielectric layer (Nexterion® HiSens slides). These innovative, next generation microarray slides were developed to identify low expressor genes, or low-abundant proteins by offering a significant increase in sensitivity over traditional transparent glass slides. The functional coating and protocol are the same as for standard slides (see “Reflective optical coating” section).






Product selection
Overview Nexterion® coatings

We have prepared a summary of all the scientific literature that cite the use of Nexterion® microarray products. The publications have been categorized by the type of slide coating used, probes printed and application. This is a useful guide when deciding which of the Nexterion® coatings to use with any particular application.

A download of this file is available here.
Alternatively, the following summary table indicates the most appropriate slide coating for specific microarray applications (Nexterion® MTP 96-well, MPX 16-well or optically coated HiSens version of the recommended Nexterion® coating are also available):
Probe type Functional coating Nexterion® coating Comments
Oligonucleotides Epoxysilane E First choice for all types of oligonucleotide arrays
Aminosilane AStar/A+ Popular option for oligonucleotide arrays
3-D thin film H or P Suitable for short amino-modified oligos (≤ 25 mers)
cDNA/PCR Aminosilane AStar/A+
Epoxysilane E
Aldehydesilane AL Alternative for amino-modified cDNA/PCR probes
Bacterial artificial chromosomes (BAC) 3-D thin film P Use with amino-modified BACs
Aminosilane
Epoxysilane E
Aldehydesilane AL Use with amino-modified BACs
Peptides 3-D thin film P Compatible with DMSO print buffer
Epoxysilane E
Aldehydesilane AL Alternative for robust peptide probes
Nitrocellulose NC
Proteins Nitrocellulose NC Optimal preservation of protein structure functionality
3-D thin film H or P Can be used with HiSens coatings
Antibodies Epoxysilane E
Aldehydesilane AL
Nitrocellulose NC
3-D thin film H
Cells/Tissues 3-D thin film H or P Optimal environment for cells and tissues
Cell lysates Nitrocellulose NC High loading capacity


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Barcoding


Laser and label barcode on Nexterion® slides
Nexterion® coated slides are available with or without a barcode. The types of barcodes available are either a label barcode or a special laser bonded foil barcode.
The barcodes are fully compatible with commercial automated hybridization stations, and are robust enough to withstand standard hybridization and washing procedures.
The barcodes conform to code 128, and are readable with all commonly available microarray scanners and hand-held barcode readers.


Packaging

Nexterion® coated slides are packed in convenient 25 or 30-slide containers for high throughput applications. The boxes are made of a specially developed plastic material to minimize out-gassing, and maintain the slide coating properties. The slide boxes are sealed in tough protective laminated foil pouches under an inert atmosphere.
The specially developed packaging protects the slides from damage due to breakage and external contamination. It also offers protection from the adverse effects of light and humidity during transportation and long-term storage.


5-slide box
25-slide box
Box for 5 MTP plates or 30 slides
Laminated foil pouch with an inert atmosphere to provide a protective environment




Frequently asked questions
General
Printing

Blocking

Hybridization
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nexterion_general_coated_2009_us.pdf Download pdf file 'General information coated slides'
Contact

Microarray Solutions
SCHOTT North America Inc.

5530 Shepherdsville Road
Louisville, KY 40228
USA
 +1 -502-657-4411
 +1 -502-966-4976
E-mail
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