General information coated slides

Introduction

SCHOTT offers a wide range of functional coating chemistries for DNA and protein microarraying.
NEXTERION® coated slides are available with a standard functional coating, or also with an additional reflective dielectric layer (NEXTERION® HiSens slides). These innovative, next generation microarray slides were developed to identify low expressor genes, or low-abundant proteins by offering a significant increase in sensitivity over traditional transparent glass slides. The functional coating and protocol are the same as for standard slides (see “Reflective optical coating” section).
Nexterion® coatings
Barcoding
packaging
Cited publications
Frequently asked questions

Product selection
Overview NEXTERION® coatings

We have prepared a summary of all the scientific literature that cite the use of NEXTERION® microarray products. The publications have been categorized by the type of slide coating used, probes printed and application. This is a useful guide when deciding which of the NEXTERION® coatings to use with any particular application.

A download of this file is available here.
Alternatively, the following summary table indicates the most appropriate slide coating for specific microarray applications (NEXTERION® MTP 96-well, MPX 16-well or optically coated HiSens version of the recommended NEXTERION® coating are also available):
Probe type Functional coating NEXTERION® coating Comments
Oligonucleotides Epoxysilane E First choice for all types of oligonucleotide arrays
Aminosilane A+ Popular option for oligonucleotide arrays
3-D thin film H Suitable for short amino-modified oligos (≤ 25 mers)
cDNA/PCR Aminosilane A+
Epoxysilane E
Aldehydesilane AL Alternative for amino-modified cDNA/PCR probes
Bacterial artificial chromosomes (BAC) Aminosilane A+
Epoxysilane E
Aldehydesilane AL Use with amino-modified BACs
Peptides Epoxysilane E
Aldehydesilane AL Alternative for robust peptide probes
Proteins 3-D thin film H Ideal substrate for printing protein
Antibodies Epoxysilane E
Aldehydesilane AL
3-D thin film H
Cells/Tissues 3-D thin film H Optimal environment for cells and tissues
Glycans 3-D thin film H First Choice



Barcoding

Different barcodes on Nexterion® microarray slides
NEXTERION® standard black laser barcode
NEXTERION® coated slides are available with or without a barcode. The standard barcode is a special laser bonded foil barcode printed inverse on the bottom side of the slide.
The barcode is fully compatible with commercial automated hybridization stations, and are robust enough to withstand standard hybridization and washing procedures.
The barcode conforms to code 128, and is readable with all commonly available microarray scanners and hand-held barcode readers.
Custom designed barcodes including logos and / or fiducial marks can also be produced. Please contact us for your special request.



Packaging

NEXTERION® coated slides are packed in convenient 25 or 30-slide containers for high throughput applications. The boxes are made of a specially developed plastic material to minimize out-gassing, and maintain the slide coating properties. The slide boxes are sealed in tough protective laminated foil pouches under an inert atmosphere.
The specially developed packaging protects the slides from damage due to breakage and external contamination. It also offers protection from the adverse effects of light and humidity during transportation and long-term storage.

Nexterion® 5 slide mailer box for storage microarray slides
5-slide box
Nexterion® 25 slide mailer box for storage microarray slides
25-slide box
Nexterion® 30 slide/5 plate mailer box for storage microarray slides or plates
Box for 5 NEXTERION® plates or 30 slides
NEXTERION® microarray slides sealed in laminated foil pouch
Laminated foil pouch with an inert atmosphere to provide a protective environment



Frequently asked questions

General
Printing

Blocking

Hybridization
Exhibitions & Events
21.
March
Exhibition Workshop "Microarrays: Basics, Production and Processing", ETH Zurich / University of Zurich, Switzerland, 03-21 to 03-22-2017