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Three-dimensional polymer coating


NEXTERION polymer coated microarray slides
SCHOTT specifically developed NEXTERION® Slide P as a dedicated slide surface for printing low density antibody microarrays. NEXTERION® Slide P produces excellent signal-to-background ratios, and exceptionally wide dynamic ranges compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. The permeable, polymer coating has a large immobilization capacity, and helps to preserve and protect the antibody structure, thus ensuring high specificity and affinity. The robust coating matrix is fully compatible with commercial microarray printers and scanners. A simple printing and processing protocol is available making NEXTERION® Slide P easy to use.


Type of coating Immobilization method Typical probes Ordering information
NEXTERION® product Barcode option Item number Slides per pack
3-D polymer surface Amine reactive chemistry

Covalent binding
Antibodies Slide P Laser 1167904 25
Key product features
• Optimised substrate for printing low density antibody microarrays
• High probe loading capacity
• Extremely low non-specific binding characteristics
• Superior performance for analysis of serum samples
• High probe loading capacity
• Compatible with all common microarray printers and scanners
Typical applications
• Validation of serum protein profiles
• Target / biomarker identification
• Proteome expression profiling
• Target / biomarker validation
• Protein expression profiling
• Oncoproteomic profiling
• Cytokine quantification
• Profiling disease states
• Sandwich assays
Suitable probe types
• Polyclonal antibodies
• Monoclonal antibodies
• Recombinant antibodies
• Recombinant antibody fragments (scFab, scFv, etc.)

Immobilization chemistry

Coating chemistry of NEXTERION polymer coated microarray slides
The coating on the SCHOTT NEXTERION® Slide P is a three-dimensional, hydrophilic polymer activated with N-Hydroxysuccinimide (NHS) esters to provide covalent immobilisation of amine groups. All NEXTERION® microarray slides are manufactured from a high quality, low-fluorescence glass coated with low-fluorescence coatings. However the non-specific binding of assay components, especially from serum samples, still remains an important contributor to the off feature background for many microarray applications. For most types of slide coatings the post-print processing protocol involves a method of adsorptive blocking to reduce non-specific binding; however these procedures are difficult to perform in a consistent manner.
The NEXTERION® Slide P coating has been engineered to exhibit an extremely low intrinsic non-specific background without the need for blocking. This was achieved by using a special polymer that is extremely resistant to non-specific binding. During in-house tests run by SCHOTT, NEXTERION® Slide P had the lowest background signal of any microarray slide coating ever tested. The polymer coating has a three-dimensional structure; with NHS-ester reactive groups attached to the hydrophilic polymer layer. The surface-exposed amino-groups on the antibodies react immediately and irreversibly with the NHS-ester groups forming covalent bonds. The polymer coating maintains the immobilised antibodies in a quasi-liquid environment that maintains their functionality and specificity. The polymer, combined with the end-point attachment, orients the immobilized bio-molecules away from the glass facilitating the interactions of the attached antibodies with the target antigens in the assay sample.

Product details

Highly reproducible coating
NEXTERION® Slide P is fabricated using a proprietary deposition process developed by SCHOTT to produce a uniform and reproducible polymer coating on one side of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the absence of particle contamination before and after coating. The coating is applied in a tightly controlled, Class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence.

Excellent spot morphology and signal-to-background ratios
NEXTERION® Slide P provides excellent spot morphologies and reproducible spot sizes over a wide range of probe concentrations.
Spot morphology of NEXTERION polymer coated microarray slides
Figure shows scanned images of NEXTERION® Slide P evaluated in an anti-IgG/IgG interaction study over a range of probe concentrations dissolved in NEXTERION® Spot PB and a phosphate print buffer.

The NEXTERION® Slide P coating is relatively hydrophilic, and care must be taken to ensure that the spot-to-spot pitch distance is set high enough to avoid spot merging.
Printing buffer Spot diameter
(split pins, SMP3)
NEXTERION® Spot PB 160 µm
50% DMSO, borate pH 8.0 90 µm
Packaging and storage
NEXTERION® Slide P are packaged in chemically stable plastic boxes and sealed under an inert atmosphere. The slides are ready-to-use from the box, and are stable for up to 12 months in the sealed packaging when stored at –20ºC. .

NEXTERION® Slide P is available in packs of 25-slides with Code 128 barcodes enabling automated sample tracking. The identical P coating is also available in either 16-well, 48-well or 96-well formats. For further information refer to the section “Multi-well formats”.

Compatible reagents

Protocol step Recommended NEXTERION® products Alternatives Recommended concentrations
Spotting NEXTERION® Spot PB 150 mM Phosphate, pH 8.5, 5 % Glycerol, 0.1 mg/ml BSA, 0.01% Sarcosyl or Tween 20®

1× phosphate-buffered saline (PBS) (Gibco/Invitrogen) pH 7.4 with 0.5% Tween 20®

1× phosphate-buffered saline (PBS) (Gibco/Invitrogen) pH 7.4 with 0.5% trehalose (added immediately before printing)
<Protein concentration 0.1 to 1 mg/mL
Chemical deactivation of unreacted NHS-esters - 50 mM ethanolamine in 50 mM sodium borate buffer pH 8.0 – 9.0
Incubation - 137mM NaCl, 2.7mM KCl, 4.3Mm Na2HPO4, 1.4mM KH2PO4, pH 7.5 with 0.5% Tween20®


NEXTERION® Slide P: Protein application
Protocol revision
Date: January 2011
Protocol version: 1.3
Revision made: Protocol optimized for printing and processing antibody arrays
Revision reason: To make protocol application specific

Date: April 2009
Protocol version: 1.2
Revision made: New telephone and fax numbers
Revision reason: Update contact information

Date: August 2008
Protocol version: 1.1
Revision made: Changed Tween® concentration (incubation step), pH of blocking solution
Revision reason: Optimization of blocking and incubation
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