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SCHOTT North America
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Nexterion® Slide H
Overview |
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Technical Information
for Protein
microarraying
[more]
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Technical Information
for DNA
microarraying
[more]
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Short Profile
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Nexterion® Slide H is ideally suited for covalent immobilization of peptides
and proteins such as antibodies, antibody fragments, enzymes, or receptors.
In addition, amino-modified oligonucleotides (size ≤ 25 mers) can also be
immobilized on the slide surface. The permeable, 3D hydrogel coating
preserves the native three-dimensional structure of proteins thereby maintaining
protein stability and functionality. Nexterion® Slide H enables excellent
signal-to-background ratios and exceptionally high linear dynamic
ranges compared to conventional 2D coatings by a unique combination of
low non-specific binding characteristics, high loading capacity, and the use
of glass with low auto-fluorescence. Even very low intensity signals, such as
those from low-abundance proteins or weakly expressed genes can be reliably
detected and quantified. The density of the reactive groups is consistent
over the entire surface and is optimized to maximize binding capacity. The
reactive chemistry is stable and remains active even during very long spotting
runs.
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| Key Product Features |
| • Ideal substrate for spotting peptide, protein, or cell/tissue microarrays |
| • High loading capacity |
| • Very low non-specific binding characteristics |
| • Optimal preservation of native structure and biological activity of protein probes |
| • High signal intensities even from low abundant proteins or weakly
expressed genes |
| • Compatible with common microarrayers (contact and non-contact) and
scanners |
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| Recommended Probe Types |
| • Antibodies and antibody fragments
(scFV or Fab) |
| • Functional proteins such as enzymes
or receptors |
| • Small protein fragments such as
peptides |
| • Cells and tissues |
| • Short amino-modified oligonucleotides
(size ≤ 25 mers) |
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Business Contact |
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Microarray Solutions
SCHOTT North America Inc.
5530 Shepherdsville Road
Louisville, KY 40228
USA
 +1 -502-657-4417
 +1 -502-966-4976
 E-mail
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 more contacts |
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