General product information
High quality borosilicate glass – The solid foundation behind NEXTERION® Microarray Slides from SCHOTTSCHOTT Microarray Solutions has exclusive access to 1.0 mm thick BOROFLOAT® 33 glass (Glass B) for the NEXTERION® microarray coated substrates. BOROFLOAT® 33 is a borosilicate glass that offers high chemical resistance, low fluorescence, and excellent flatness. The glass is precision cut into the microscope slide format for standard NEXTERION® slides and other formats, such as the NEXTERION® MTP-96 microplate. All slides are laser-cut to create straight robust edges. Additional information on different glass types and more detailed specifications can be found in the section “Uncoated slides”.
BOROFLOAT® 33 glass exhibits excellent chemical stability and durability. The glass provides an inert support for biomolecule immobilization, and hybridization, and does not leach alkali ions over time. For this reason, borosilicate glasses are highly suited to microarraying applications.
The high-purity borosilicate glass demonstrates extremely low and uniform fluorescence at the Cy3™ and Cy5™ (570 nm and 670 nm) emission wavelengths. The low fluorescence of the glass offers exceptionally low background signals during the scanning of a microarray, thereby maximizing the signal-to-background ratios. Consequently, even very low signal intensities, such as those from weakly expressed genes, or low abundance proteins, can be reliably detected.
Flatness is an extremely important characteristic for microarray slides, as microarrays are typically printed with pins that have to come into contact with the slide surface to deposit probes. BOROFLOAT® 33 is manufactured by a float glass process to produce an exceptional flatness of ≤50 μm and a high quality surface finish. "Flatness" is defined by SCHOTT as the accumulated overall possible thickness deviation. This includes warp, intra-slide thickness deviation, and inter-slide thickness tolerance.
All uncoated and coated slides are cut to size using an innovative laser system to obtain precise, and highly accurate cut edges with no micro-cracks. A laser beam precisely heats the glass followed by a jet of cold liquid. This thermally induced tension causes a fissure in the glass. This results in the highest possible quality cut, in terms of edge quality and strength. Laser cut edges have a high strength that resists subsequent fragmentation or chipping. This helps to ensure the microarray slide surface remains free of particle contamination.
edge of a laser cut slide
edge of a conventional slide