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NEXTERION^® blocking reagents

NEXTERION® Block A Kit

Easy-to-use reagent for deactivating aminosilane coating after printing NEXTERION® Block A is a highly effective reagent for blocking aminosilane slide surfaces after microarray printing. It is ideal for use with both NEXTERION® Slide A+ and Slide AStar, as well as other commercial slides. The reagent is based on the well accepted BSA blocking method, and works by blocking the reactive groups on the slide surface not occupied by the spotted DNA molecules. This significantly reduces any potential non-specific binding of the labeled target, thereby minimizing the background signals on the final microarray. The reagent is supplied as a convenient, pre-made, two-part solution that does not contain any hazardous materials. order code: 100 mL 1206704, 500 mL 1206717 concentration: 1 x

NEXTERION® Block E

Highly effective reagent for deactivating epoxy coating after printing NEXTERION® Block E is a blocking solution optimized for NEXTERION® Slide E. It reacts rapidly with residual epoxy groups in both the printed and unprinted areas of the slide surface and was developed to ensure high signal intensities with reduced non-specific background, thus increasing overall data reproducibility. SCHOTT Microarray Solutions has demonstrated that NEXTERION® Block E is an extremely important component for producing high quality DNA microarrays with very good signal-to-background ratios. NEXTERION® Block E is available as a 4x concentration in 100 and 1000 mL packs. order code: 100 mL 1066069, 1000 mL 1066071 concentration: 4 x

NEXTERION® Oligo Pre-Hyb

A time-saving reagent combining slide blocking and washing steps NEXTERION® Oligo Pre-Hyb is an effective reagent for blocking epoxysilane slide surfaces after microarray printing. The NEXTERION® Oligo Pre-Hyb offers the advantage combining some of the initial washing and blocking steps, thereby reducing the overall processing time. It is ideal for use with both NEXTERION® Slide E, and other commercially available Epoxide slides printed with 20 to 70 mer oligonucleotides. The reagent is supplied as a convenient, ready-to-use solution. order code: 1000 mL 1116889 ready-to-use

MSDS

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Frequently asked questions

What is the recommended method of preparing NEXTERION® Block E? What is the pH of the NEXTERION® Block E before, and after adding the required water and hydrochloric acid? How stable is the diluted NEXTERION® Block E? Can I leave out the blocking steps for NEXTERION® Slide AStar and E? I want to store my slides after printing; but before hybridization. Should I store them directly after printing, or after the washing and blocking steps? How can I avoid bleeding spots or comet tails? In the DNA application protocol for NEXTERION® Slide AL, you recommend a "chemical deactivation" step with Sodium borohydride (NaBH4). This does not appear in the protein application protocol, and seems to be replaced by a blocking step. Is the chemical deactivation and blocking the same?