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NEXTERION^® hybridization buffers

NEXTERION® Hyb

Formamide free hybridization buffer NEXTERION® Hyb is a hybridization buffer that has been developed for optimal spot morphology, high signal intensities with reduced non-specific background, and high data reproducibility on NEXTERION® slides. NEXTERION® Hyb is compatible with many different surface chemistries and hybridization methods and may be used with cDNA or oligonucleotide arrays. The reagent is supplied as a ready-to-use 1x concentrated solution. By avoiding the laborious preparation of multi-component hybridization buffers, NEXTERION® Hyb can save users time and effort. The components in the buffer help to stabilize the hybridization process during extended runs, and also reduce background fluorescence. NEXTERION® Hyb has a low-viscosity, does not contains formamide and is recommended for applications that cannot tolerate formamide, or that require temperatures greater than 60 ºC. NEXTERION® Hyb is available as a 1x concentration in 100 mL packs. order code: 1066075 concentration: 1 x

NEXTERION® Oligo Hyb

Formamide hybridization buffer for oligonucleotides arrays NEXTERION® Oligo Hyb has been optimized for use with epoxy coated (NEXTERION® Slide E) slides printed with oligonucleotides 20 to 70 mers long. NEXTERION® Oligo Hyb is formulated with formamide. Formamide reduces the melting temperature of DNA-DNA hybrids, and permits users to reduce the hybridization temperature (42 ºC) while maintaining stringency. The combination of formamide and lower hybridization temperature dramatically reduces evaporation during hybridization. NEXTERION® Oligo Hyb is available at a ready-to-use concentration in 100 mL packs. order code: 1116890 ready-to-use

Material Safety Data Sheets

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Frequently asked questions

Which factors affect hybridization? What is the difference between NEXTERION® Hyb (order code: 1066075) and NEXTERION® Oligo Hyb (order code: 1116890)? How can I formulate my own hybridization buffer? Does the pre-hybridization buffer need to contain formamide? What can I check if there is no signal on the substrate after hybridization? What are the causes of an uneven hybridization pattern across an array? How can I decrease high background fluorescence observed after hybridization? Can SCHOTT recommend any alternatives to the Cy5 dye?