NEXTERION® hybridization buffers
Formamide free hybridization buffer
NEXTERION® Hyb is a hybridization buffer that has been developed for optimal spot morphology, high signal intensities with reduced non-specific background, and high data reproducibility on NEXTERION® slides. NEXTERION® Hyb is compatible with many different surface chemistries and hybridization methods and may be used with cDNA or oligonucleotide arrays. The reagent is supplied as a ready-to-use 1x concentrated solution. By avoiding the laborious preparation of multi-component hybridization buffers, NEXTERION® Hyb can save users time and effort. The components in the buffer help to stabilize the hybridization process during extended runs, and also reduce background fluorescence. NEXTERION® Hyb has a low-viscosity, does not contains formamide and is recommended for applications that cannot tolerate formamide, or that require temperatures greater than 60 ºC. NEXTERION® Hyb is available as a 1x concentration in 100 mL packs.
order code: 1066075
concentration: 1 x
Formamide hybridization buffer for oligonucleotides arrays
NEXTERION® Oligo Hyb has been optimized for use with epoxy coated (NEXTERION® Slide E) slides printed with oligonucleotides 20 to 70 mers long. NEXTERION® Oligo Hyb is formulated with formamide. Formamide reduces the melting temperature of DNA-DNA hybrids, and permits users to reduce the hybridization temperature (42 ºC) while maintaining stringency. The combination of formamide and lower hybridization temperature dramatically reduces evaporation during hybridization. NEXTERION® Oligo Hyb is available at a ready-to-use concentration in 100 mL packs.
order code: Formulation will be provided on request
Please request the MSDS by completing the form.
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