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SCHOTT North America
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Nexterion® hybridization buffers
Nexterion® Hyb
(order code: 1066075)
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Formamide free hybridization buffer
Nexterion® Hyb is a hybridization buffer that has been developed for optimal
spot morphology, high signal intensities with reduced non-specific background,
and high data reproducibility on Nexterion® slides. Nexterion® Hyb is compatible
with many different surface chemistries and hybridization methods and
may be used with cDNA or oligonucleotide arrays. The reagent is supplied
as a ready-to-use 1x concentrated solution. By avoiding the laborious preparation
of multi-component hybridization buffers, Nexterion® Hyb can save
users time and effort. The components in the buffer help to stabilize the
hybridization process during extended runs, and also reduce background
fluorescence. Nexterion® Hyb has a low-viscosity, does not contains formamide
and is recommended for applications that cannot tolerate formamide,
or that require temperatures greater than 60 ºC. Nexterion® Hyb is available
as a 2x concentration in 100 mL packs.
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Nexterion® Oligo Hyb
(order code: 1116890)
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Formamide hybridization buffer for oligonucleotides arrays
Nexterion® Oligo Hyb has been optimized for use with epoxy coated
(Nexterion® Slide E) slides printed with oligonucleotides 20 to 70 mers long.
Nexterion® Oligo Hyb is formulated with formamide. Formamide reduces
the melting temperature of DNA-DNA hybrids, and permits users to reduce
the hybridization temperature (42 ºC) while maintaining stringency. The
combination of formamide and lower hybridization temperature dramatically
reduces evaporation during hybridization. Nexterion® Oligo Hyb is available
at a ready-to-use concentration in 100 mL packs.
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Contact
Microarray Solutions
SCHOTT North America Inc.
5530 Shepherdsville Road Louisville, KY 40228 USA
 | +1 -502-657-4431 |
 | +1 -502-966-4976 |
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