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Nexterion® hybridization buffers

Nexterion® Hyb
(order code: 1066075)

Formamide free hybridization buffer
Nexterion® Hyb is a hybridization buffer that has been developed for optimal spot morphology, high signal intensities with reduced non-specific background, and high data reproducibility on Nexterion® slides. Nexterion® Hyb is compatible with many different surface chemistries and hybridization methods and may be used with cDNA or oligonucleotide arrays. The reagent is supplied as a ready-to-use 1x concentrated solution. By avoiding the laborious preparation of multi-component hybridization buffers, Nexterion® Hyb can save users time and effort. The components in the buffer help to stabilize the hybridization process during extended runs, and also reduce background fluorescence. Nexterion® Hyb has a low-viscosity, does not contains formamide and is recommended for applications that cannot tolerate formamide, or that require temperatures greater than 60 ºC. Nexterion® Hyb is available as a 2x concentration in 100 mL packs.
Nexterion® Oligo Hyb
(order code: 1116890)
Formamide hybridization buffer for oligonucleotides arrays
Nexterion® Oligo Hyb has been optimized for use with epoxy coated (Nexterion® Slide E) slides printed with oligonucleotides 20 to 70 mers long. Nexterion® Oligo Hyb is formulated with formamide. Formamide reduces the melting temperature of DNA-DNA hybrids, and permits users to reduce the hybridization temperature (42 ºC) while maintaining stringency. The combination of formamide and lower hybridization temperature dramatically reduces evaporation during hybridization. Nexterion® Oligo Hyb is available at a ready-to-use concentration in 100 mL packs.






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Microarray Solutions
SCHOTT North America Inc.

5530 Shepherdsville Road
Louisville, KY 40228
USA
 +1 -502-657-4411
 +1 -502-966-4976
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