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SCHOTT North America
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Nexterion® kits
Nexterion® Epoxy Slide Oligo
Processing Kit
(order code: 1209009)
(for 25 slides)
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SCHOTT Microarray Solutions offers a kit with pre-prepared reagents for the
blocking, hybridization and washing of 25 printed epoxysilane coated slides.
The reagents in the kit are optimized for use with epoxy coated slides (e.g.
Nexterion® Slide E) printed with 20 to 70 mer oligonucleotides. The Nexterion®
Oligo Pre-Hyb offers the advantage combining some of the initial washing
and blocking steps thereby reducing the overall processing time. Nexterion®
Oligo Hyb contains formamide, which allows for increased hybridization
stringency at decreased hybridization temperatures. The kit includes an easyto-
use slide processing protocol.
Superior signal-to-background ratios
Enhanced signal-to-background ratios were observed over the entire dynamic
range (as shown by the results from low, medium, and high expressor genes)
when 70 mer oligonucleotides were spotted onto the Nexterion® Slide E.
The covalent probe binding properties of the slide surface allow the optimal
presentation of the probe and therefore improved target accessibility for
hybridization. In addition, the reactive groups on the slide surface can be
fully deactivated using a blocking reagent greatly reducing the background
fluorescence caused by non-specific binding.
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The graph shows normalized signal-tobackground
ratios (S/Bk) for the market
leading aminosilane slide and Nexterion®
Slide E. Signal-to-background ratios
were calculated for three selected genes
with different expression levels (low,
medium and high). To allow the presentation of all the data in one graph, all
the results were normalized against the values obtained for the market leading
aminosilane slide.
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The Epoxy Slide Oligo Processing Kit contains the following components:
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| Process step |
Components |
Reagent
concentration |
Quantity |
| Blocking |
Nexterion® Oligo
Pre-Hyb |
Ready-to-use
1x |
1 x 500 mL |
| Hybridization |
Nexterion® Oligo
Hyb |
Ready-to-use
1x |
1 x 10 mL |
| Washing |
Nexterion® Oligo
Hyb |
20x |
1 x 1000 mL |
| Nexterion® Oligo
Wash B |
20x |
1 x 100 mL |
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Nexterion® Aminosilane Slide
Processing Kit
(order code: 1209008)
(for 25 slides)
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SCHOTT Microarray Solutions also offers a kit for 25 printed aminosilane
coated slides (e. g. Nexterion® Slide AStar/A+). The Nexterion® Block A offers
the advantage of combining some of the initial washing and blocking steps
thereby reducing the overall processing time. Nexterion® Oligo Hyb contains
form amide, which allows for increased hybridization stringency at decreased
hybridization temperatures. The kit includes an easy-to-use slide processing
protocol.
The Aminosilane Slide Processing Kit contains the following components:
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| Process step |
Components |
Reagent
concentration |
Quantity |
| Blocking |
Nexterion® Block A |
Ready-to-use
1x |
1 x 500 mL |
| Hybridization |
Nexterion® Oligo
Hyb |
Ready-to-use
1x |
1 x 10 mL |
| Washing |
Nexterion® Wash A |
20x |
1 x 1000 mL |
| Nexterion® Wash B |
20x |
1 x 100 mL |
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Nexterion® “Active” Slide
Chemistry Evaluation Kits
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To assist customers evaluating the Nexterion® slide surfaces, SCHOTT offers
ready-to-use slide evaluation kits for two of its most commonly used slide
surfaces. The Nexterion® Slide Evaluation Kits offer users the opportunity to
fully evaluate the slide performance by utilizing the optimized protocol and
ready-to-use reagents included in the kit. The kit consists of ten slides with
test oligonucleotides, optimized reagents and detailed protocols.
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Components |
Quantity |
| Microarray Slide |
Nexterion® Slide E |
10 slides |
| Spotting |
Nexterion® Spot
Nexterion® Spot LE Kit
(Low Evaporation Buffer) |
10 mL
10 mL |
| Blocking |
Nexterion® Block E |
100 mL |
| Hybridization |
Nexterion® Hyb |
10 mL |
| Test probe |
Oligo probe |
2 nmol |
| Test target |
Oligo target |
3 pmol |
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Components |
Quantity |
| Microarray Slide |
Nexterion® Slide AL |
10 slides |
| Spotting |
Nexterion® Spot |
10 mL |
| Hybridization |
Nexterion® Hyb |
10 mL |
| Test probe |
Oligo probe |
2 nmol |
| Test target |
Oligo target |
3 pmol |
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Contact
Microarray Solutions
SCHOTT North America Inc.
5530 Shepherdsville Road Louisville, KY 40228 USA
 | +1 -502-657-4431 |
 | +1 -502-966-4976 |
E-mail
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